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Programme

Thursday 10 Nov

08:30

Registration

09:00

Opening/Welcome

René Leemans/Andreas Dietz/Wojciech Golusinski

Epidemiology and carcinogenesis

Chairs: Andreas Dietz/Wojciech Golusinski

09:20

Worldwide incidence of HPV in OPSCC

Pawel Golusinski

09:40

HPV-mediated carcinogenesis

Ruud Brakenhoff

Head and neck squamous cell carcinomas (HNSCC) develops in the mucosal lining of the upper aerodigestive tract. The tumours are generally preceded by precancerous changes in the mucosal epithelium. Some of these are macroscopically visible as lesions, but most can only be identified in surgical margins by microscopic examination for dysplasia or by genetic analysis. As these mucosal changes are often not macroscopically visible, they may stay behind untreated and cause recurrent disease.

Since two decades it became recognized that human papillomavirus infection causes a separate subgroup of oropharyngeal cancers. These tumours have a more favourable prognosis and during subsequent years it became apparent that one of the reasons for this favourable prognosis was the lack of precancerous fields preceding the carcinomas. This is highly remarkable as in cervical carcinogenesis, these HPV-mediated tumours are preceded by mucosal lesions that can be visualized using acetic acid, and are the main target of the population-based screening programs for cervical cancer.

Most information on HPV-mediated carcinogenesis has been obtained from cervical cancer research, and remarkable similarities between the putative target cells for malignant transformation, have been found. The lack of HPV-associated precancerous changes in the upper-aerodigestive tract, however, remains an enigma. In this presentation the various aspects of HPV-mediated carcinogenesis will be addressed, including a model and some novel insights.

Ruud Brakenhoff

Ruud Brakenhoff

10:00

Mucosal stem cells and HPV-infection

Jochen Hess

10:20

Coffee

10:50

Treatment of HPV disease with (chemo)radiotherapy

Vincent Gregoire

11:10

Proffered papers

Clinical Presentation and Outcome of HPV-positive Nasopharyngeal Carcinoma in a North American Cohort; Shao Hui Huang

Single cell RNA Sequencing allows mapping of HPV transcripts in head and neck cancer epithelial cells; Cornelius Kürten

Awareness of HPV-associated oropharyngeal cancer among the general population and general practitioners in The Netherlands: cross-sectional study; Femke Verhees

HPV16 sequence variants and prognosis in HPV-positive OPSCC; Irene Nauta

Prognosis and serology HPV in OPSCC

Chairs: Ruud Brakenhoff/ Maura Gillison

11:50

Validation of statistical models for OPSCC

Robert Baatenburg de Jong

12:10

Subclassification of HPV-OPSCC by gene expression

Lisa Licitra

12:30

HPV early antigen serology and OPC screening

Tim Waterboer

12:45

Antibody producing B-cells in HPV+ve OPSCC: an overview

Rieneke van de Ven

Rieneke van de Ven

Rieneke van de Ven

13:00

Lunch

13:30

Satellite symposium HPV as target for treatment

Combination immunotherapy of cancers caused by high risk HPV16
Anna-Sophia Wiekmeijer, Isa Pharmaceuticals

Therapeutic DNA-enabled Immunotherapies for the treatment of HPV diseases
Jeffrey Skolnik, Senior Vice President of Clinical Development, Inovio

Arenaviral Vectors Impacting HPV16+ HNSC
Klaus Orlinger and Corrine Iacobucci, Hookipa Biotech GmbH

Treatment of HPV+ve OPSCC

Chairs: René Leemans/Hans Langendijk

14:30

Immunotherapy for HPV driven disease

Maura Gillison

14:50

Treatment of HPV disease by laser surgery

Terry Jones

15:10

Immunotherapy for HPV driven disease

Maura Gillison

15:30

Proffered papers

The course of health-related quality of life from diagnosis to two years follow-up in patients with oropharyngeal cancer: does HPV status matter? Laura H.A. Korsten

Ninety-day Mortality following TORS or Definitive Radiotherapy for Oropharyngeal Cancer at American Commission on Cancer-Accredited Facilities; James Bates

Detection and Monitoring of Circulating Tumor HPV DNA in HPV-Associated Sinonasal and Nasopharyngeal Cancers; Daniel Faden

16:00

Coffee

Chairs: Christian Simon/Stephan Lang

16:30

Are there alternatives for 3 weekly cisplatin in HPV disease?

Jan Vermorken

16:50

Salvage surgery for HPV disease

Carole Fakhry

Debate

17:10

Debate: This house believes that TNM8 was implemented too early for HPV+ve OPSCC

For: Hisham Mehanna / Against: Brian O’Sullivan

17:00

Posters and Drinks

19:00

Social evening (dinner)

Friday 11 Nov

Future perspectives in HPV research

Chairs: Rieneke van de Ven/Ingeborg Tinhofer-Keilholz

09:00

Proffered papers

Differences between HPV positive and HPV negative oropharyngeal cancer detected by Non-Gaussian IVIM; Iris Lauwers

Loss of LRP1B expression drives chemo and radiation resistance in HPV-positive head and neck cancer; Mushfiq Shaikh

Proximity ligation-based sequencing for mapping viral integration sites in human papillomavirus positive head and neck squamous cell carcinomas; Imke Demers

09:30

HPV in non-oropharygeal HNSCC: does it play a role?

Gunnar Wichmann

09:50

Therapeutic HPV vaccination

Sjoerd van der Burg

HPV16+ OPSCC patients display an overall longer survival and a lower recurrence rate after standard of care treatment than HPV- patients.  Because the composition of the tumor immune microenvironment (TIME) associated with good prognosis generally also predicts the success of immunotherapy, we started  to analyze an unique prospective cohort of HPV- and HPV+  OPSCC patients. We found that clinical outcome is strongly linked to the presence of HPV-specific CD4+ and CD8+ tumor infiltrating lymphocytes (TIL) during >10 years follow-up. New evidence, suggests that immunity is not only targeted at the E6 and E oncoproteins but may also involve the E2 protein. Subsequently, we performed a comprehensive multimodal, high-dimensional strategy to dissect the TIME of treatment-naïve IR+ and IR− OPSCC tissue, including bulk RNA sequencing, imaging mass cytometry and combined single-cell gene expression profiling and T-cell receptor of TIL. This revealed that IR+ tumors expressed higher levels of genes strongly related to interferon gamma signaling, T-cell activation, TCR-signaling, and mononuclear cell differentiation, as well as several immune signaling pathways, than IR− patients, specifically related to ectopic lymphoid structure development. Moreover, scRNAseq identified a subset of clonally expanded CD8+ T cells, dominantly present in IR+ tumors, which secreted T-cell and DC-attracting chemokines. Immune cell infiltration in IR+ tumors is stronger, highly coordinated, and has a distinct spatial phenotypical signature characterized by intra-tumoral microaggregates of CD8+CD103+ and CD4+ T-cells with DCs. In contrast, the IR− TIME comprised spatial interactions between lymphocytes and various immunosuppressive myeloid cell populations, which differed per patient. The impact of these results are discussed in view of our previous and current trials with (combinations of) therapeutic vaccination and checkpoint strategies.

Sjoerd van der Burg

Sjoerd van der Burg

Head Of Research Laboratories, Senior Investigator Oncode Institute

Therapy developments in HPV+ve OPSCC

Chairs: Jan Vermorken/Vincent Gregoire

10:10

De-escalation of non-surgical therapy

Hisham Mehanna

10:30

Coffee

11:00

Proffered papers

Role of the PuraBond haemostatic agent in Trans oral Robotic surgery – A case series; Mriganka De

Early detection of recurrent HPV-positive HNSCC by ctDNA analysis; Annouk Pierik

11:20

Treatment of HPV disease by transoral robotic surgery

Christian Simon

11:40

Is proton therapy preferable for HPV+ve OPSCC?

Hans Langendijk

12:00

Tumor board (moderator: Terry Jones)

Wojciech Golusinski, Stephan Lang, Hans Langendijk, Lisa Licitra, Bob Ferris

13:00

Lunch

Therapy developments in HPV+ve OPSCC

Chairs: Lisa Licitra/Hans Langendijk

14:00

Cell therapy for HPV-driven disease

Christian Hinrichs

14:20

De-escalation using surgery

Bob Ferris

14:40

Proffered papers

Deciphering Knowledge and Opinions of HPV and HPV Vaccination for Facilitation of Point-of-Care Vaccination; Daniel Faden

Nodal involvement determines the initial quantity of circulating free HPV16 DNA in patients with HPV-related oropharyngeal cancer; Tomasz Rutkowski

The tumor immune microenvironment of HPV-negative and HPV-positive oropharynx squamous cell carcinomas; Tara Muijlwijk

Screening and Prevention

Chair: Andreas Dietz

15:10

Round table screening and prevention

Discussion leader: Andreas Dietz

5-slide Pitch Hans Berkhof: Preventive vaccination, also in treated OPSCC patients?
5-slide Pitch Tim Waterboer: HPV serology for screening
5-slide Pitch Ingeborg Tinhofer-Keilholz: HPV DNA assays for screening
5-slide Pitch Carole Fakhry: Positively screened for HPV: which intervention?

Hans Berkhof

Hans Berkhof

16:00

Round table discussion: screening and prevention

16:30

Summary

Wojciech Golusinski

16:40

Abstract/Poster awards and closing remarks

René Leemans

16:50

Adjourn